EST-PAGE--managing and analyzing EST data

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EST-PAGE - managing and analyzing EST data

UNLABELLED EST-PAGE provides a bioinformatics solution for expressed sequence tags (EST) data entry, database management, GenBank submission, process control and data retrieval from a unified web interface that can be easily customized and adapted by groups working on diverse EST sequencing projects. AVAILABILITY The system and source code are available upon request from the authors. SUPPLE...

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ApiEST-DB: analyzing clustered EST data of the apicomplexan parasites

ApiEST-DB (http://www.cbil.upenn.edu/paradbs-servlet/) provides integrated access to publicly available EST data from protozoan parasites in the phylum Apicomplexa. The database currently incorporates a total of nearly 100,000 ESTs from several parasite species of clinical and/or veterinary interest, including Eimeria tenella, Neospora caninum, Plasmodium falciparum, Sarcocystis neurona and Tox...

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Limitations of the EST Data

Figure A Overview of Steps Involved in a Cot Analysis. (A) Nuclear DNA is sheared into 200-500 bp fragments by high-speed blending, and (B) fragment size is checked by gel electrophoresis. (C) Sheared DNA is precipitated, dissolved in 0.03, 0.12, or 0.5 M sodium phosphate buffer (SPB), and aliquots are loaded/sealed into glass microcapillary tubes/ampoules. (D) One tube/ampoule is (E) placed in...

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EST Pipeline System: Detailed and Automated EST Data Processing and Mining

Expressed sequence tags (ESTs) are widely used in gene survey research these years. The EST Pipeline System, software developed by Hangzhou Genomics Institute (HGI), can automatically analyze different scalar EST sequences by suitable methods. All the analysis reports, including those of vector masking, sequence assembly, gene annotation, Gene Ontology classification, and some other analyses, c...

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ژورنال

عنوان ژورنال: Bioinformatics

سال: 2004

ISSN: 1367-4803,1460-2059

DOI: 10.1093/bioinformatics/btg411